WebFluorescein is a fluorophore commonly used in microscopy, in a type of dye laser as the gain medium, in forensics and serology to detect latent blood stains, and in dye tracing. … WebFluorescein arsenical hairpin binder (FlAsH): a nonfluorescent molecule but becomes highly fluorescent upon binding to a specific sequence consisting of a biarsenical-tetracysteine tag with the form Fluorescence resonance energy transfer (FRET): an optical technique that measures changes in intramolecular conformations or –protein ...

Fluorescent labeling of tetracysteine-tagged proteins in

Webon fluorophores which can attach to proteins in several sites positions: Fluorescein arsenical hairpin binder (FlAsH) contains, for instance, a biarsenical group that can bind to a tetracysteine motif Cys-Cys-Pro-Gly-Cys-Cys in proteins [22]. This label has been used to study orientational dynamics of a G-protein-coupled receptor in WebHowever, the large size and spectral properties of FP impose certain limitations for their use. The recently developed Fluorescein Arsenical Hairpin (FlAsH/tetracysteine) binder technology emerged as a promising alternative to FP for protein labeling and cellular localization studies. shurflo revolution

(PDF) Application of Intramolecular FlAsH-BRET to the …

WebMar 15, 2015 · Using the established concept of FIAsH-EDT2 based binding with cysteine tagged biomolecules that result in fluorescence ‘ON or OFF’ system, we aim to establish a generic nanoswitch that can be used for multiple applications. WebIn this paper, we provide a general protocol for labeling proteins with the membrane-permeant fluorogenic biarsenical dye fluorescein arsenical hairpin … WebHere we use G protein-coupled receptor (GPCR) activation in living cells as a model system to compare YFP with the small, membrane-permeant fluorescein derivative with two arsen-(III) substituents (fluorescein arsenical hairpin binder; FlAsH) targeted to a short tetracysteine sequence. shurflo raw water strainer