Gfp hepg2 cells

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WebApr 12, 2024 · CES2 inhibition in a human liver cell line (HepG2) phenocopies Ces2a deficiency. ... 609 bp and WT Ces2a: 1030 bp. For adenoviral overexpression of CES2 or GFP, mice were fed HFD for 20 weeks and then injected with 1.6 x 109 PFU particles of adenoviral vectors (Vector Biolabs) into the tail vein [8]. Six days post-injection, a … WebHepG2-preS1-GFP cells were cultured in R10 culture medium as follows. 1. Prepare R10 medium by adding to RPMI 1640 10% of heat inactivated fetal bovine serum, 20 mM HEPES, 1 mM sodium pyruvate, 100 IU/mL penicillin, 100 μg/mL streptomycin, MeM amino acids, Glutamax, MeM non-essential amino acids, 5 μg/mL Plasmocin. 2.

High-content imaging-based BAC-GFP toxicity pathway …

WebNov 11, 2024 · FX binding was evaluated by preincubating Ad-GFP-Luc virus with media containing either buffer or physiological levels of blood coagulation factor X for 10 min at 37 °C (10 μg/mL; Haematologic Technologies; HCX-0050) before infecting confluent HepG2 cells at 500vp/cell and evaluating transduction by flow cytometry as described above. WebMay 26, 2015 · The expression of yieF was measured in stably transfected cells HepG2-YieF by quantitative RT-PCR and found up-regulated by 3.89-fold upon Cr(VI) induction. … henry\u0027s fork fishing

HepG2 hepatoma reporter cells InvivoGen

WebIn this study, the hypoglycemic activity of a polysaccharide obtained from Grifola frondosa (GFP) on HepG2 cell and hpyerglycemic mechanism were investigated. The purity of the isolated polysaccharides was examined by HPLC. In this research, it was found that GFP enhanced the absorption of glucose of HepG2 cells in a dose dependent manner at 24 ... WebMar 25, 2024 · g, Live-cell imaging of HEPG2 cells that were incubated at 37 °C for 1 h with 50 nM rabbit IgG-647 and 25 nM goat anti-rabbit, goat anti-rabbit M6Pn or goat anti-rabbit GalNAc. Scale bar, 20 µm ... WebOct 14, 2014 · Cells successfully transfected with GFP were separated by flow cytometry (FACScan; Becton Dickinson, San Jose, CA), and the purified, GFP-positive cell lines were named HepG2-199a and HepG2-NC (NC, negative control). ... MTT assays showed that HepG2 cells with increased miR-199a expression (HepG2-199a) proliferated at a slower … henry\u0027s fork anglers discount

Construction of EGFP Expressing HepG2 Cell Line Using ... - Springer

Hepatocyte polarity establishment and apical lumen formation …

WebApr 14, 2024 · B HepG2 cells were transfected for 24 h with the indicated combinations of Flag control, Flag-SESN2, Flag-SESN2 and Flag-IGF2BP3-GFP vectors and further cultured in the absence of glucose (Glc ... WebBottom: HepG2 cells were transfected with a plasmid expressing GFP, and visualized using either visible light or fluorescence. Experiment 3: Comparison of EndoFectin™ HepG2 and electroporation EndoFectin™ HepG2 can also provide nearly as high transfection efficiency as electroporation, meaning that you can achieve ultra-high levels of ... henry\u0027s fork anglers guidesWebEach reagent was used to transfect HEK 293, HeLa, LNCaP, HepG2, and A549 cell lines in a 96-well format, and GFP expression was analyzed 48 hours post-transfection. Lipofectamine 3000 reagent provided higher GFP transfection efficiency than Lipofectamine 2000 and FuGENE HD reagents for all five cell lines. Figure 2. henry\u0027s fork

"WebTo investigate the molecular mechanism underlying the inhibitory effects of TMEM2 on HBV infection in HepG2 and HepG2.2.15 cells, western blot was used to evaluate the … " - Gfp hepg2 cells

Gfp hepg2 cells

IJMS Free Full-Text Chromate Reductase YieF from Escherichia …

WebHepG2-Dual™ cells are designed to allow the simultaneous study of the NF-κB and IRF pathways by measuring the activity of SEAP and Lucia luciferase, respectively. HepG2 … WebHepG2, HepG2/GFP and HepG2/GFP-HBx cells were treated with adriamycin (2.5 microg/ml), and apoptosis of the cells was determined by their morphological changes, …

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WebHepG2 Cell Line Origin and Characteristics. HepG2 is an immortalized cell line consisting of human liver carcinoma cells, derived from the liver tissue of a 15-year-old Caucasian … WebDec 15, 2016 · Livecell-imaging microscopy analysis of HBV Myr-preS1-AX594 binding and endocytosis in NTCP-GFP expressing HepG2-cells. HepG2-cells were transiently transfected with an expression plasmid encoding for hNTCP-GFP fusion-protein (green).After seeding and 2 days of culturing in Ibidi-chamberslides, cells were …

WebHepG2 cells are adherent, epithelial-like cells growing as monolayers and also in small aggregates. About turboGFP protein: tGFP is an improved variant of the green fluorescent protein CopGFP from copepoda … WebAug 28, 2024 · Thereafter culture medium was exchanged and HepG2 or HSC Col-GFP cells were either left for a further 24 h and the supernatant was used for co-immunoprecipitation (HepG2, Section 2.12) or after 10 h medium was exchanged and cells were starved for 16 h and stimulated the next day (HepG2, HSC Col-GFP, Section 2.5).

WebJul 5, 2024 · E GFP expression in HepG2-NTCP cells transfected with GFP-encoding plasmids under the control of either a CMV-IE promoter or an EF1α promoter and subsequently cultured in indicated medium for indicated times. Scale bar … WebHCT 116. CCL-247 ™. HCT 116 cell line was isolated from the colon of an adult male with colon cancer. It has a mutation in codon 13 of the ras proto-oncogene and can be used as a positive control for PCR assays of mutation in this codon. Product category.

WebApr 19, 2024 · HepG2 cells were infected with the GFP-LC3 adenovirus. After 24 h, the cells were starved in Earle’s balanced salt solution (EBSS, Thermo Fisher Scientific, Waltham, MA, USA, 14155063) for 4 h and treated with ZZY-19 (10 μM), sorafenib (10 μM), or ZZY-19 plus sorafenib for 24 h.

WebJul 8, 2024 · All the BAC-GFP HepG2 reporter cell lines (p53-GFP, MDM2-GFP, and p21-GFP) were previously established and characterized . The cells were used between passage 14 and 20 and seeded in Greiner black μ-clear 384 well plates, at 8000 cells per well. Immunostaining. Western blot. henry\\u0027s fork anglersWebGeneral Transfection Protocols Cell-Specific Transfection Protocols Transfection Reagents - Product Protocols NOTE: Invitrogen Lipofectamine Transfection Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially in a new cell line. henry\u0027s fork fly fishingWebAn optimized electroporation protocol was developed for transfection of HepG2 cells stably expressing green fluorescent protein. Electric pulses of 600 V/cm yielded the highest transfection efficiency and stably transfected clones were selected after 14 days incubation in selection medium. henry\\u0027s fork fishing reportWebApr 14, 2024 · B HepG2 cells were transfected for 24 h with the indicated combinations of Flag control, Flag-SESN2, Flag-SESN2 and Flag-IGF2BP3-GFP vectors and further cultured in the absence of glucose (Glc ... henry\u0027s fork foundation ashton idahoWebExperimental result: HepG2 cells were successfully transfected with pcDNA3.1-GFP plasmid using X-tremeGENE™ 9 DNA Transfection Reagent. Transfection of Neuro-2a Cells. Protocol provided by a customer. Plate Neuro-2a cells at a … henry\u0027s fork fishing reportsWebJan 27, 2015 · To make stably NTCP overexpressed HepG2, I used G418 selection vector, pcDNA3.1 (+). Three days after transfection NTCP-FLAG-pcDNA3.1 (+) to HepG2, I conducted immunofluorescence and I confirmed ... henry\\u0027s fork fly fishingWebTransfection efficiency of TransfeX Reagent on HepG2. HepG2 cells were prepared and seeded at 2.5 x 105 cells/well in a 12 well plate at 98% viability. HepG2 cells were transfected with EF1α-eGFP vector at 1.0 µg DNA with 2.0 µL of reagent (1:2) in Opti-MEM I Reduced Serum Media. No significant signs of cellular toxicity were observed. Phase ... henry\\u0027s fork foundation