Phospho-beclin-1
WebPhospho-Beclin-1 (Ser90) (E3X1I) Rabbit mAb recognizes endogenous levels of Beclin-1 protein only when phosphorylated at Ser90. This antibody may not recognize Beclin-1 when dually phosphorylated at Ser90 and Ser93. Species Reactivity: Human Source / Purification WebA phosphotyrosine antibody was used for phospho-BECLIN-1 and phospho-BCR-ABL detection. (B) Immunoprecipitation of BCR-ABL with BECLIN-1 and (C) BECLIN-1 with ABL in K562 cells confirms the interaction reciprocally and at endogenous level. (D) GST-pull-down of BCR-ABL with recombinant GST-Beclin-1 in K562 cells corroborated the interaction ...
Phospho-beclin-1
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Web在本研究中,ROT 通过增加 DEPTOR 水平和 p -/t-AMPK 比率。此外,ROT 通过降低 p-/t-ERK1/2 比率和 beclin-1 水平来增加 p-/t-Akt 比率和糖原合酶激酶-3β (GSK3β) 活性。ROT 还通过在 Triton X-100 可溶性和不溶性细胞裂解物部分中诱导自噬体形成来促进 LC3B-I 脂化为 … WebRabbit Anti-Human Phospho-beclin 1(S64) Antibody, 400 ul
WebProduct overview. Phospho-Beclin 1 (S234) ELISA Kit (ab279747) is a very rapid, convenient, and sensitive assay kit that can monitor the activation or function of important biological pathways in human cell lysates. By determining phosphorylated Beclin 1 protein in your experimental model system, you can verify pathway activation in your cell ... WebOct 27, 2024 · These results clearly exhibited that Beclin 1 was phosphorylated at Ser90 and the antibody for phosphorylated Beclin 1 at Ser90 was specific. Ionomycin, EB1089, and Vitamin D3, three...
WebMay 26, 2024 · Beclin 1 protects cells from necroptosis in an autophagy-independent manner. Beclin 1 is a modulator of the apoptosis pathway [].To further identify whether … WebThe Beclin-1 S14 phosphorylation by ULK is required for full autophagic induction in mammals and this requirement is conserved in C. elegans. Our study reveals a molecular link from ULK1 to activation of the autophagy specific VPS34 …
WebSpecificity / Sensitivity. Phospho-Beclin-1 (Ser93/96) Antibody recognizes overexpressed levels of Beclin-1 protein only when phosphorylated at Ser93 and Ser96 (Ser91 and Ser94 …
WebWestern blot - Phospho-Beclin 1-Ser15 Rabbit pAb (AP1252) Western blot analysis of extracts of various cell lines, using Phospho-Beclin 1-Ser15 antibody (AP1252) at 1:500 dilution.NIH/3T3 and C6 cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L ... how far away is spearfish south dakotaWebThe effects on the PI3K/Akt/mTOR axis (PI3K, Akt, phospho-Akt, mTOR, and phospho-mTOR) during FeHV-1 infection were examined in Western blot analysis. We found an increase in … how far away is spicer mn from meWebPhospho-Beclin-1 (Ser93) (D9A5G) Rabbit mAb recognizes endogenous levels of Beclin-1 protein only when phosphorylated at Ser93. This antibody recognizes single phosphorylation of Ser93 to a greater extent than dual phosphorylation at Ser93 and Ser96. This antibody may also weakly detect an unidentified band at approximately 70 kDa. hiding knowledge in workplaceWebBeclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser234. Beclin 1 pS234 Antibody is ideal for researchers interested in cancer research. Application Note. Anti-Phospho-Ser234 Beclin-1 Antibody is tested for WB. Expect a band at approximately ~60 kDa on specific lysates or tissues. how far away is spain from mexicoWebAnti-Beclin1 (Ser295) Antibody. Our Anti-Beclin1 (Ser295) rabbit polyclonal phosphospecific primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, … hiding joe rogan baggy clothesWebWestern Blot analysis of Phospho-Beclin 1 (Ser90) in various cell lines using a Phospho-Beclin 1 (Ser90) Polyclonal antibody ( Product # PA5-121359) at a dilution of 1:500. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary ... how far away is springfieldWebNov 16, 2012 · Here, we show that Beclin 1, an essential autophagy and tumor suppressor protein, is a target of the protein kinase Akt. Expression of a Beclin 1 mutant resistant to … hiding items games